FD – BUG: Microbial fuel tests


Test system for diesel fuel microbial issues.

Test for bacteria & bugs in the field or in the workshop.

Test kit components

1 x test kit for bacterial & algae problems.

1 x test kit for fungus (moulds & yeasts).

Other components

1 x Instructions & analysis charts.

1 x syringe for loading sample on test plates.

2 x clear bags for storing test plates when incubating.

1 x set gloves.

Test kit suitable for all types of diesel fuel including dyed and bio fuels.

contact us to order


Microbial diesel fuel test kit for bacteria & fungus

Suitable for all types of diesel fuel testing applications

  • marine – power generation – construction equipment
  • automotive – storage tanks – transport equipment + more

Easy to use – Test results in 24 to 48 hours

The test kit provides non-quantitative results as your first steps in evaluating diesel fuel for present or developing microbial problems.

Takes 24 to 48 hours for results @ temperature of 24°C to 35°C / 77°F to 95°F.


1 x test plate for fungus (moulds & yeasts) detection.

1 x test plate for bacteria detection.

1 x syringe for loading fuel sample onto test plates.

1 x set gloves.

Misc. components

1 x Instructions.

No warranties or guarantees are made regards the accuracy of the test results that you may interpret from using this kit.

If more definitive & accurate tests are required or for critical application fuel testing use a laboratory for a complete quantitative analysis.

This test kit contains no liquids, no toxic or hazardous materials.

FD – BUG testing steps: microbial growth fuel tests

Remove the test 2 plates from packets when ready to start, remove the covers on the plates, use the syringe provided to place 1 ml of fuel onto the centre of each test plate agar, the fuel will absorb out over the agar media area.

Refit the plate covers, then insert test plates into their original foil packing bags or store them in the clear bag we provide or store in any container for incubation. Store the test plates with the lid side of the test plate upwards at all times.

Incubation steps:

Once you have completed the above steps you will need to store the plates for the incubation period at a temperature of between 24°C to 35°C / 77°F to 95°F for 24 to 48 hours.

Do not place the plates into direct sunlight to increase temperature.

If you cannot incubate within or close to these temperatures needed you will require an incubation device to achieve the optimum temperatures required – we have inexpensive incubator devices for purchase if required.

Depending on your incubation temperature and the levels of microbial growth in your fuel you may see visual signs of some problems from about 24 hours of incubation.

Should you have signs of growth at this early stage you already have positive results that you have microbial issues in your fuel samples.

Once incubated for the required times and temperatures, compare the test plates to the images on the instructions sheet provided for identifying any problems.

  • Test plate C fungus: this test will indicate mould and yeast problems.
  • Test plate D bacteria: this test will indicate bacterial problems.

Place white paper under the test plate for better viewing. You can view the test results from the top or bottom of the plate which ever side is clearer to view. Any signs of growth indicates signs of bugs in your fuel.

If your fuel fails testing, you many need additives to stabilize / destroy microbial bugs in your fuel, or you may need to undertake fuel servicing / cleaning to get your fuel & system back to peak operating condition.

Fuel bugs are not considered a health hazard to humans or animals. To neutralize any microbial residue before disposing of plates just drop some bleach onto agar area.